欧阳明星 博士
Email：mxouyang@cczu.edu.cn
欧阳明星，男，博士，教授，1976年生。2005年在中国科学院生物物理研究所博士毕业。2006年起先后在伊利洛伊大学香槟分校（UIUC）和加州理工学院（Caltech）做博士后；2011年起先后在耶鲁大学（Yale）和加州大学圣地亚哥分校（UCSD）做助理研究员；2017年加入常州大学，2019年入选江苏省特聘教授。在生物医学工程领域有10多年的研究经验，重点方向是细胞分子生物学和生物力学，在显微镜活细胞成像和荧光共振能量转移技术（FRET）领域累积了丰富的经验。
已发表论文30多篇，SCI引用1200多次，专利两项，主要一作/并列一作的论文发表在Nature Communications, PNAS (2008, 2012), Cancer Research, J Biol. Chem., ACS Sensors，ACS Biomaterials Sci & Eng等杂志上，研究工作主要包括FRET分子探针研发及应用，如理解细胞信号转导和分子的生物物理调控等机制；生物力学领域，细胞迁移中力学调控信号的极性分布及机制，长程生物力作用的研究（PNAS，2012）具有一定突破性，发现细胞之间存在远距离的力学交流，得到同时期Nature Materials和PNAS杂志的报道。目前主持国家面上、省面上、省特聘人才类项目。
主要研究方向
生物力学/力生物学、细胞分子生物学、FRET技术
教育、科研工作经历
2017.09－ 教授 常州大学，生物医学工程与健康科学研究院
2013.08－2015.10 助理研究员 加州大学圣地亚哥分校 (UCSD)，生物工程系
2011.09－2013.07 助理研究员 耶鲁大学医学院 (Yale)，心血管中心
2009.07－2011.08 博士后 加州理工学院 (Caltech)，生物工程系
2006.01－2009.06 博士后 伊利洛伊大学香槟分校 (UIUC)，生物工程系
1999.09－2005.06 硕博连读 中国科学院生物物理研究所，生物物理专业
1995.09－1999.06 本科 四川大学，生物系生态专业
主要科研成果：
1. 1. Mingxing Ouyang#, *, Yiming Zhu#, Jiajia Wang, Qingyu Zhang, Yanling Hu, Bing Bu, Jia Guo, Linhong Deng* (2022) Mechanical communication-associated cell directional migration and branching connections mediated by calcium channels, integrin β1 and N-cadherin. Front. Cell Dev. Biol. fcell.2022.942058.
2. 2. Xin Fang, Kai Ni, Jia Guo, Yaqin Li, Ying Zhou, Hui Sheng, Bing Bu, Mingzhi Luo, Mingxing Ouyang*, Linhong Deng* (2022) FRET visualization of cyclic stretch-activated ERK via calcium channels mechanosensation while not integrin β1 in airway smooth muscle cells. Front. Cell Dev. Biol.fcell.2022.847852.
3. 3. Mingxing Ouyang*, Jiunyann Yu, Yenyu Chen, Linhong Deng, Chin-Lin Guo* (2021) Cell-extracellular matrix interactions in the fluidic phase direct the topology and polarity of self-organized epithelial structures. Cell Proliferation, 54(4):e13014.
4. Mingxing Ouyang#,*, Zhili Qian#, Bing Bu, Yang Jin, Jiajia Wang, Lei Liu, Yan Pan, Linhong Deng* (2020) Sensing Traction Force Induces Cell-Cell Distant Mechanical Communications for Self-assembly. ACS Biomaterials Sci & Eng 6, 5833−5848.
5. Jiajia Wang, Jia Guo, Bo Che, Mingxing Ouyang*, Linhong Deng* (2020) Cell motion-coordinated fibrillar assembly of soluble collagen I to promote MDCK cell branching formation. Biochem Biophy Res Commun 524: 317-324.
6. Hui Yao, L. W., Jia Guo, Weimin Liu, Jingjing Li, Yingxiao Wang, Linhong Deng* and Mingxing Ouyang*. (2020) Genetically Encoded FRET Biosensor Detects the Enzymatic Activity of Prostate-Specific Antigen. Molecular & Cellular Biomechanics DOI:10.32604/mcb.2020.09595.
7. Mingxing Ouyang, Rongxue Wan, Qin Qin, Qin Peng, Pengzhi Wang, Jenny Wu, Molly Allen, Yiwen Shi, Shannon Laub, Linhong Deng, Shaoying Lu*, Yingxiao Wang* (2019) Sensitive FRET biosensor reveals Fyn kinase regulation by sub-membrane localization. ACS Sensors 4(1): 76-86.
8. Mingxing Ouyang#, Shaoying Lu#, Yingxiao Wang* (2014) Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of MT1-MMP Protease Activity. Methods in Molecular Biology 1071: 163-74. doi: 10.1007/978-1- 62703-622-1_13.
9. Mingxing Ouyang#, Shaoying Lu#, Chin-En Chen, Taejin Kim, Deborah Leckband, Fei Wang, Albert B. Reynolds, Martin A. Schwartz*, Yingxiao Wang* (2013) N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signaling pathways. Nature Communications, 4:1589. doi: 10.1038/ncomms2560.
10. Chin-Lin Guo*,#, Mingxing Ouyang#, Jiun-Yann Yu, Jordan Maslov, Andrew Price, and Chih-Yu Shen (2012) Feature Article: Long-range mechanical force enables self-assembly of epithelial tubular patterns. Proc Natl Acad Sci USA. 109(15):5576-82.
同行报道和评述:
“Secrets of tubule engineering by epithelial cells.” Commentary in Proc Natl Acad Sci USA. (2012) 109 (18): 6790- 6791.
“Morphogenesis: Laying down the tracks.” News and Views in Nature Materials (2012) 11, 490-492.
11. Mingxing Ouyang#, He Huang#, Nathan Shaner, Albert Remacle, Sergey Shiryaev, Alex Strongin, Roger Y. Tsien, Yingxiao Wang* (2010) Simultaneous Visualization of Pro-tumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer. Cancer Research 70(6):2204-12.
12. Mingxing Ouyang, Jie Sun, Shu Chien* & Yingxiao Wang* (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with novel FRET biosensors. Proc Natl Acad Sci USA 105(38): 14353-8.
13. Mingxing Ouyang, Shaoying Lu, Xiao-Yan Li, Jing Xu, JihyeSeong Ben N. G. Giepmans, John Y.-J. Shyy, Stephen J. Weiss, and Yingxiao Wang* (2008) Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Activity in Live Cells by Fluorescence Resonance Energy Transfer (FRET) Imaging. J Biological ChemistryVol. 283, 17740- 17748.
14. Mingxing Ouyang, Xun Shen* (2006) Critical role of ASK1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma SH-SY5Y cells. J Neurochemistry, Vol. 97, 234-244. (注释：#一作或并列一作；* 通信作者)
15. Wenjie Huang, Meiling Tan, Yue Wang, Lei Liu, Yan Pan, Jingjing Li, Mingxing Ouyang, Xiangping Qu, Huijun Liu, Chi Liu, Linhong Deng, Yang Xiang, Xiaoqun Qin (2020) Increased intracellular Cl- concentration improves airway epithelial wound repair via activating RhoA/ROCK Pathway.Theranostics10(19):8528-8540. doi: 10.7150/thno.46002.
16. Ziliang Huang, Mingxing Ouyang, Shaoying Lu, Yingxiao Wang*, Qin Peng* (2020) Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. Journal of Molecular Biology 432(7): 1901-1909.
17. Qin Qin, Shannon Laub, Yiwen Shi, Mingxing Ouyang, Qin Peng, Jin Zhang, Yingxiao Wang, Shaoying Lu* (2019) Fluocell for Ratiometric and High-Throughput Live-Cell Image Visualization and Quantification. Frontiers in Physics 7: 154. doi.org/10.3389/fphy.2019.00154.
18. Rongxue Wan, Jenny Wu, Mingxing Ouyang, Lei Lei, Jiaming Wei, Qin Peng, Reed Harrison, Yiqian Wu, Binbin Cheng, Kaitao Li, Cheng Zhu, Liling Tang*,Shaoying Lu*, Yingxiao Wang* (2019) Biophysical Basis underlying Dynamic Lck Activation Visualized by ZapLck FRET Biosensor. Science Advances, 2019;5: eaau2001.
19. Pengzhi Wang#, Jing Liang#, Linda Z. Shi, Yi Wang, Ping Zhang, Mingxing Ouyang, Daryl Preece, Qin Peng, Lunan Shao, Jason Fan, Jie Sun, Shawn S. Li, Michael W. Berns1, Huimin Zhao* and Yingxiao Wang* (2018) Visualizing spatiotemporal dynamics of intercellular mechanotransmission upon wounding. ACS Photonics, 5(9): 356576.
20. Jie Sun, Lei Lei, Chih-Ming Tsai, Yi Wang, Yiwen Shi, Mingxing Ouyang, Shaoying Lu, JihyeSeong, Tae-Jin Kim, Pengzhi Wang, Min Huang, Xiangdong Xu, Victor Nizet, Shu Chien*, Yingxiao Wang* (2017) Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nature Communication8(1):477. doi: 10.1038/s41467-017-00569-6.
21. Abhishek Kumar, Mingxing Ouyang, Koen Van den Dries, Ewan James McGhee, Keiichiro Tanaka, Marie D. Anderson, Alexander Groisman, Benjamin T. Goult, Kurt I. Anderson, and Martin A. Schwartz (2016) Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. Journal of Cell Biology 213(3):371-83.
22. Chen Sun, Mingxing Ouyang, Zhenning Cao, Sai Ma, Hamzeh Alqublan, Nammalwar Sriranganathan, Yingxiao Wang and Chang Lu (2014) Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chem. Commun., 50: 11536-39 DOI: 10.1039/C4CC04730C.
23. Shaoying Lu, JihyeSeong, Yi Wang, Shiou-chi Chang, John Paul Eichorst, Mingxing Ouyang, Julie Y.-S. Li, Shu Chien & Yingxiao Wang. (2014) Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells. Scientific Report 4:5756. doi: 10.1038/srep05756.
24. Bo Liu, Shaoying Lu, Ying-li Hu, Xiaoling Liao, Mingxing Ouyang&Yingxiao Wang (2014) RhoA and Membrane Fluidity Mediates the Spatially Polarized Src/FAK Activation in Response to Shear Stress. Scientific Reports 4, Article No. 7008 (2014).
25. Chin-Lin Guo, Mingxing Ouyang, Jiun-Yann Yu (2013) Long-range mechanical force enables self-assembly of epithelial tubules. Mechanics of Biological Systems and Materials, Volume 5, Chapter 3:15-21.
26. Tyler D. Ross, Brian G. Coon, SangeokYoun, Nicolas Baeyens, Keiichiro Tanaka, Mingxing Ouyang, Martin A. Schwartz. Integrins in Mechanotransduction. Current Opinions in Cell Biology 2013 Oct; 25(5):613-8. doi: 10.1016/j.ceb.2013.05.006.
27. Jie Sun, Shaoying Lu, Mingxing Ouyang, Li-Jung Lin, Yue Zhuo, Bo Liu, Shu Chien, Benjamin G. Neel, Yingxiao Wang. (2013) Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2. Nature Communications 4:2037. doi: 10.1038/ncomms3037.
28. Jiun-Yann Yu, Chun-Hung Kuo, Daniel B. Holland, Yenyu Chen, Mingxing Ouyang, Geoffrey A. Blake, Ruben Zadoyan,and Chin-Lin Guo (2011) Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy. J Biomed Opt 16(11):116009.
29. JihyeSeong, Mingxing Ouyang, Taejin Kim, Jie Sun, Po-Chao Wen, Shaoying Lu, Yue Zhuo, Nicholas M. Llewellyn, David D. Schlaepfer, Jun-Lin Guan, Shu Chien&Yingxiao Wang (2011) Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2:406. doi: 10.1038/ncomms1414.
30. Shaoying Lu, Tae-jin Kim, Chih-En Chen, Mingxing Ouyang, Yingxiao Wang. (2011) Computational Analysis and Visualization for the Spatiotemporal Coordination of Molecular Activities in Live Cells. PLoS One 6(6):e21293.
31. KatarzynaGawlik, Albert G. Remacle, Sergey A. Shiryaev, Vladislav S. Golubkov, Mingxing Ouyang, Yingxiao Wang, Alex Y. Strongin (2010) A femtomol range FRET biosensor reports exceedingly low levels of cell surface furin: implications for the processing of anthrax protective antigen. PLoS One 5(6):e11305.
32. Yeh-ChuinPoh, Sungsoo Na, Farhan Chowdhury, Mingxing Ouyang, Yingxiao Wang, Ning Wang (2009) Rapid Activation of RacGTPase in Living Cells by Force Is Independent of Src. Plos One, 4(11):e7886.
33. Sungsoo Na, Farhan Chowdhury, Bernard Tay, Mingxing Ouyang, Martin Gregor, Yingxiao Wang, Gerhard Wiche, and Ning Wang (2009) Plectin contributes to mechanical properties of living cells. Am J Physiol Cell Physiol 296(4):C868-77.
34. JihyeSeong, Shaoying Lu, Mingxing Ouyang, He Huang, Jin Zhang, Margaret C. Frame and Yingxiao Wang (2009) Visualization of Src Activity at Different Compartments of the Plasma Membrane by FRET Imaging. Chem Biol 16(1):48-57.
35. Tae-jin Kim, JihyeSeong, Mingxing Ouyang, Shaoying Lu, Jie Sun, Jun Pyu Hong, Ning Wang, Yingxiao Wang (2009) Mechanical rigidity regulates Ca2+ oscillation via RhoA signaling pathway in human mesenchymal stem cells. J Cell Physiol 218(2):285-93.
36. Shaoying Lu, Mingxing Ouyang, JihyeSeong, Jing Zhang, Shu Chien, Yingxiao Wang (2008) The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging. PLOS Comput Biol 4(7): e1000127.
37. Sungsoo Na, Olivier Collin, Farhan Chowdhury, Bernard Tay, Mingxing Ouyang,Yingxiao Wang, and Ning Wang (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc Natl Acad Sci USA Vol. 105, No. 18, 6625-6631.
同行杂志的报道：“Faster than a Speeding Molecule,” Science Signaling (2008) 1, ec175.
发明专利
38. Yingxiao Wang, Mingxing Ouyang. “Detection of Specific Binding Reactions using Magnetic Labels”, Patent No.: US 8,192,947 B2.
39. Yingxiao Wang, Shaoying Lu, Mingxing Ouyang. “FRET-Based Membrane Type 1 Matrix Metalloproteinase Biosensors and Methods for Using the Same”, Patent No.: US 2011/0171675 A1.