欧阳明星

发布者:董进 发布时间:2018-09-07 浏览次数:894

欧阳明星 博士
Email:
mxouyang@cczu.edu.cn


      欧阳明星,博士,教授。 2005年在中国科学院生物物理研究所博士毕业。2006年起先后在伊利洛伊大学香槟分校(UIUC)和加州理工学院(Caltech)做博士后,在耶鲁大学(Yale)和加州大学圣地亚哥分校(UCSD)做助理研究员。在生物工程等领域有10多年的研究经验,重点方向是细胞生物学、分子生物学和生物力学,在显微镜活细胞成像(live cell imaging)和荧光能量共振传导技术(FRET)领域累积了丰富的经验。在SCI期刊发表论文20多篇,其中发表主要一作和并列一作研究论文的杂志包括Nature Communication, PNAS(2篇),Cancer Research, J Biological Chemistry,和 J Cell Biology(二作)以及2项专利(二作和三作)。


主要研究领域
生物工程、细胞生物学、分子生物学、生物力学、FRET技术


教育、科研工作经历
2017.09-                    教授               常州大学,生物医学工程与健康科学研究院
2013.08-2017.08     助理研究员         加州大学圣地亚哥分校,生物工程系(Assistant Project Scientist, 2014年1月起)
2011.09 -2013.07    助理研究员         耶鲁大学医学院,心血管研究中心
2009.07-2011.08        博士后             加州理工学院,生物工程系
2006.01 -2009.06       博士后             伊利洛伊大学香槟分校,生物工程系
1999.09 -2005.06    硕博研究生          中国科学院生物物理研究所,生物物理专业
1995.09 -1999.06       本科生             四川大学,生物系


主要科研成果:
学术论文
  1. Mingxing Ouyang*, Shaoying Lu*, Yingxiao Wang# (2014) Genetically Encoded Fluorescent  Biosensors for Live-cell Imaging of MT1-MMP Protease Activity. Methods in Molecular Biology 2014;1071:163-74. doi: 10.1007/978-1- 62703-622-1_13.

  2. Mingxing Ouyang*, Shaoying Lu*, Chin-En Chen, Taejin Kim, Deborah Leckband, Fei Wang, Albert B. Reynolds, Martin A. Schwartz#, Yingxiao Wang# (2013) N-cadherin regulates spatially polarized signals through multiple effectors: p120ctn and β-catenin determine distinct features. Nature Communication, 4:1589. doi: 10.1038/ncomms2560.

  3. Chin-Lin Guo*,#, Mingxing Ouyang*, Jiun-Yann Yu, Jordan Maslov, Andrew Price, and Chih-Yu Shen (2012) Feature Article: Long-range mechanical force enables self-assembly of epithelial tubular patterns. Proc Natl Acad Sci USA. 109(15):5576-82.
同行的报道和评述:

“Secrets of tubule engineering by epithelial cells.” Commentary in Proc Natl Acad Sci USA. (2012) 109 (18): 6790- 6791.

“Morphogenesis: Laying down the tracks.” News and Views in Nature Materials (2012) 11, 490-492.

  4. Mingxing Ouyang*, He Huang*, Nathan Shaner, Albert Remacle, Sergey Shiryaev, Alex Strongin, Roger Y. Tsien, Yingxiao Wang# (2010) Simultaneous Visualization of Pro-tumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer. Cancer Research 70(6):2204-12.

  5. Mingxing Ouyang*, Jie Sun, Shu Chien# & Yingxiao Wang# (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with novel FRET biosensors. Proc Natl Acad Sci USA 105(38): 14353-8.

  6. Mingxing Ouyang*, Shaoying Lu, Xiao-Yan Li, Jing Xu, Jihye Seong Ben N. G. Giepmans, John Y.-J. Shyy, Stephen J. Weiss, and Yingxiao Wang# (2008) Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Activity in Live Cells by Fluorescence Resonance Energy Transfer (FRET) Imaging. J Biological Chemistry Vol. 283, 17740- 17748.

  7. Mingxing Ouyang*, Xun Shen# (2006) Critical role of ASK1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma SH-SY5Y cells. J Neurochemistry, Vol. 97, 234-244.
(注释: * 一作或并列一作;#通信作者)

  8. Jie Sun, Lei Lei, Chih-Ming Tsai, Yi Wang, Yiwen Shi, Mingxing Ouyang, Shaoying Lu, Jihye Seong, Tae-Jin Kim, Pengzhi Wang, Min Huang, Xiangdong Xu, Victor Nizet, Shu Chien & Yingxiao Wang (2017) Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nature Communication 2017 Sep 7;8(1):477. doi: 10.1038/s41467-017-00569-6.

  9. Abhishek Kumar, Mingxing Ouyang, Koen Van den Dries, Ewan James McGhee, Keiichiro Tanaka, Marie D. Anderson, Alexander Groisman, Benjamin T. Goult, Kurt I. Anderson, and Martin A. Schwartz (2016) Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. Journal of Cell Biology 2016 Jul 18;214(2):231.

  10. Chen Sun, Mingxing Ouyang, Zhenning Cao, Sai Ma, Hamzeh Alqublan, Nammalwar Sriranganathan, Yingxiao Wang and Chang Lu (2014) Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chem. Commun., 2014, 50, 11536-11539 DOI: 10.1039/C4CC04730C.

  11. Shaoying Lu, Jihye Seong, Yi Wang, Shiou-chi Chang, John Paul Eichorst, Mingxing Ouyang, Julie Y.-S. Li, Shu Chien & Yingxiao Wang (2014) Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells. Scientific Report 2014 Jul 24;4:5756. doi: 10.1038/srep05756.

  12. Bo Liu, Shaoying Lu, Ying-li Hu, Xiaoling Liao, Mingxing Ouyang & Yingxiao Wang (2014) RhoA and Membrane Fluidity Mediates the Spatially Polarized Src/FAK Activation in Response to Shear Stress. Scientific Reports 4, Article No. 7008 (2014).

  13. Chin-Lin Guo, Mingxing Ouyang, Jiun-Yann Yu (2013) Long-range mechanical force enables self-assembly of epithelial tubules. Mechanics of Biological Systems and Materials, Volume 5, Chapter 3.

  14. Tyler D. Ross, Brian G. Coon, Sangeok Youn, Nicolas Baeyens, Keiichiro Tanaka, Mingxing Ouyang, Martin A. Schwartz. Integrins in Mechanotransduction. Current Opinions in Cell Biology 2013 Oct; 25(5):613-8. doi: 10.1016/j.ceb.2013.05.006.

  15. Jie Sun, Shaoying Lu, Mingxing Ouyang, Li-Jung Lin, Yue Zhuo, Bo Liu, Shu Chien, Benjamin G. Neel, Yingxiao Wang. (2013) Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2. Nature Communications, 2013;4:2037. doi: 10.1038/ncomms3037.

  16. Jiun-Yann Yu, Chun-Hung Kuo, Daniel B. Holland, Yenyu Chen, Mingxing Ouyang, Geoffrey A. Blake, Ruben Zadoyan,and Chin-Lin Guo (2011) Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy. J Biomed Opt 16(11):116009.

  17. Jihye Seong, Mingxing Ouyang, Taejin Kim, Jie Sun, Po-Chao Wen, Shaoying Lu, Yue Zhuo, Nicholas M. Llewellyn, David D. Schlaepfer, Jun-Lin Guan, Shu Chien & Yingxiao Wang (2011) Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2:406. doi: 10.1038/ncomms1414.

  18. Shaoying Lu, Tae-jin Kim, Chih-En Chen, Mingxing Ouyang, Yingxiao Wang. (2011) Computational Analysis and Visualization for the Spatiotemporal Coordination of Molecular Activities in Live Cells. PLoS One 6(6):e21293.

  19. Katarzyna Gawlik, Albert G. Remacle, Sergey A. Shiryaev, Vladislav S. Golubkov, Mingxing Ouyang, Yingxiao Wang, Alex Y. Strongin (2010) A femtomol range FRET biosensor reports exceedingly low levels of cell surface furin: implications for the processing of anthrax protective antigen. PLoS One 5(6):e11305.

  20. Yeh-Chuin Poh, Sungsoo Na, Farhan Chowdhury, Mingxing Ouyang, Yingxiao Wang, Ning Wang (2009) Rapid Activation of Rac GTPase in Living Cells by Force Is Independent of Src. Plos One, 4(11):e7886.

  21. Sungsoo Na, Farhan Chowdhury, Bernard Tay, Mingxing Ouyang, Martin Gregor, Yingxiao Wang, Gerhard Wiche, and Ning Wang (2009) Plectin contributes to mechanical properties of living cells. Am J Physiol Cell Physiol 296(4):C868-77.

  22. Jihye Seong, Shaoying Lu, Mingxing Ouyang, He Huang, Jin Zhang, Margaret C. Frame and Yingxiao Wang (2009) Visualization of Src Activity at Different Compartments of the Plasma Membrane by FRET Imaging. Chem Biol 16(1):48-57.

  23. Tae-jin Kim, Jihye Seong, Mingxing Ouyang, Shaoying Lu, Jie Sun, Jun Pyu Hong, Ning Wang, Yingxiao Wang (2009) Mechanical rigidity regulates Ca2+ oscillation via RhoA signaling pathway in human mesenchymal stem cells. J Cell Physiol 218(2):285-93.

  24. Shaoying Lu, Mingxing Ouyang, Jihye Seong, Jing Zhang, Shu Chien, Yingxiao Wang (2008) The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging. PLOS Comput Biol 4(7): e1000127.

  25. Sungsoo Na, Olivier Collin, Farhan Chowdhury, Bernard Tay, Mingxing Ouyang, Yingxiao Wang, and Ning Wang (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc Natl Acad Sci USA Vol. 105, No. 18, 6625-6631. 

杂志报道: “Faster than a Speeding Molecule,” Science Signaling (2008) 1, ec175.

发明专利
  26. Yingxiao Wang, Mingxing Ouyang. “Detection of Specific Binding Reactions using Magnetic Labels”, 专利号 PCT/US2007/081173.

  27. Yingxiao Wang, Shaoying Lu, Mingxing Ouyang. “FRET-Based Membrane Type 1 Matrix Metalloproteinase Biosensors and Methods for Using the Same”, 专利号 PCT/US2009/052713.